Description: Laboratory Protocol Safety 1 Microscopy1 Brightfield Microscopy2 Darkfield Microscopy3 Phase-Contrast Microscopy4 Fluorescence Microscopy5 Microscopic Measurements2 Survey of Microorganisms6 Protozoans, Algae, and Cyanobacteria7 Ubiquity of Bacteria8 The Fungi: Yeasts and Molds3 Manipulation of Microorganisms 9 Aseptic Technique10 Pure Culture Techniques4 Staining and Observation of Microorganisms11 Smear Preparation12 Simple Staining13 Negative Staining14 Capsular Staining15 Gram Staining16 Spore Staining: Two Methods17 Acid-Fast Staining: Ziehl-Neelsen Method18 Motility Determination5 Culture Methods19 Culture Media Preparation20 Preparation of Stock Cultures21 Cultivation of Anaerobes22 Enumeration of Bacteria: The Standard Plate Count23 Slime Mold Culture24 Slide Culture: Molds6 Bacterial Viruses25 Determination of a Bacteriophage Titer26 Burst Size Determination: A One-Step Growth Curve 27 Isolation of Phage from Flies28 Phage Typing7 Environmental Influences and Control of Microbial Growth29 Temperature: Effects on Growth30 Temperature: Lethal Effects31 pH and Microbial Growth 32 Water Activity and Osmotic Pressure33 Ultraviolet Light: Lethal Effects34 Oligodynamic Action 35 Evaluation of Alcohol: Its Effectiveness as an Antiseptic36 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method37 Evaluation of Antiseptics: The Filter Paper Disk Method38 Effectiveness of Hand Scrubbing8 Identification of Unknown Bacteria39 Morphological Study of Unknown Bacterium40 Cultural Characteristics41 Physiological Characteristics: Oxidation and Fermentation Tests42 Physiological Characteristics: Hydrolytic Reactions43 Physiological Characteristics: Biochemical Tests44 Use of Bergey's Manual9 Miniaturized Multitest Systems45 Enterobacteriaceae Identification: The API 20E System46 Enterobacteriaceae Identification: The Enterotube II System 47 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System48 Staphylococcus Identification: The API Staph-Ident System10 Diversity and Environmental Microbiology49 Isolation of an Antibiotic Producer: The Actinomyces50 The Nitrogen Cycle: Amonification51 Symbiotic Nitrogen Fixation: Rhizobium52 Free-living Nitrogen Fixation: Azotobacter53 Denitrifcation: Paracoccus denitrificans54 The Winogradsky Column 55 Purple Nonsulphur Photosynthetic Bacteria56 Sulfate Reducing Bacteria: Desulfovibrio57 Bacterial Commensalism58 Bacterial Synergism59 Microbial Antagonism11 Applied Microbiology 60 Bacterial Counts of Foods61 Bacterial Examination of Water: Qualitative Tests62 The Membrane Filter Method63 Reductase Test64 Microbial Spoilage of Canned Food65 Microbiology of Alcohol Fermentation 12 Bacterial Genetics and Biotechnology66 Mutant Isolation by Replica Plating67 Bacterial Transformation 68 Polymerase Chain Reaction for Amplifying DNA69 Plasmid Isolation13 Medical Microbiology and Immunology70 The Staphylococci: Isolation and Identification71 The Streptococci: Isolation and Identification72 Gram Negative Intestinal Pathogens 73 Slide Agglutination Test: Serological Typing 74 Slide Agglutination (Latex) Test: For S. aureus Identification75 Tube Agglutination Test: The Heterophile Antibody Test76 White Blood Cell Study: The Differential WBC Count77 Blood Grouping78 A Synthetic Epidemic Appendixes A Tables B Indicators, Stains, Reagents C Media D Identification Charts E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics

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