Description: Introduction to the Text. Objectives of the Biochemistry Laboratory. 1. Biochemistry Boot Camp. Lab Safety. Scientific Notation. Significant Figures. Statistics and Scientific Measurements. Units. Concentration of Solutions. Dilutions. Graphing. Pipets and Pipetmen. Experiment #1: Use of Pipettors. 2. Acids, Bases, and Buffers. Strong Acids and Bases. Weak Acids and Bases. Polyprotic Acids. Buffers. Good Buffers. Choosing a Buffer. Effect of Concentration and Temperature. How We Make Buffers. The Big Summary. Why Is This Important'. Expanding the Topic. Experiment #2: Preparation of Buffers. 3. Spectrophotometry. Absorption of light. Beer-Lambert Law. Standard Curves. Protein Assays. Why Is This Important'. Expanding the Topic--Calculating concentrations from graphs. Tricks of the Trade: Choosing Test Tubes and Pipets. Experiment #3: Beer's Law and Standard Curves. Experiment #3B: Protein Concentration of LDH Fractions. 4. Enzyme Purification. Enzymes as Catalysts. Enzyme Purification. Units of Enzyme Activity. Calculating Initial Velocity. Purification Tables. Assay for Lactate Dehydrogenase (LDH). Why is this important'. Tricks of the Trade. Experiment #4: Purification of LDH (short version). Experiment #4A: Purification of LDH (comprehensive version). 5. Ion Exchange Chromatography. Amino Acids as Weak Acids and Bases. Isoelectric Points. Ion Exchange Chromatography. Ion Exchange Resins. Identification of Compounds Eluted from Columns. Thin Layer Chromatography. Why Is This Important'. Experiment #5: Separation and Identification of Amino Acids. Experiment #5A: Purification of LDH with Ion Exchange Chromatography. 6. Affinity Chromatography. Affinity Chromatography. Gel Supports. Affinity Ligands. Elution of bound molecules. Why Is This Important'. Experiment #6: Affinity Chromatography of LDH. 7. Gel Filtration Chromatography. Gel Filtration. Types of Supports. Determining the Molecular Weight. Distribution Coefficients. Why Is This Important'. Expanding the Topic. Experiment #7: Gel Filtration Chromatography. Experiment #7A: Gel Filtration Chromatography of LDH. 8. Enzyme Kinetics. Reaction Tates. Order of Reactions. Michaelis-Menten Approach. Significance of Km and Vmax. Linear Plots. Properties of Tyrosinase. Why Is This Important'. Experiment #8: Enzyme Kinetics of Tyrosinase. Experiment #8A: Enzyme Kinetics of LDH. 9. Electrophoresis. Electrophoresis. Agarose Gels. Polyacrylamide Gels. SDS. Staining Gels. Lactate Dehydrogenase. Why Is This Important'. Expanding the topic. Experiment #9: Native Gel Separation of LDH Isozymes (short version). Experiment #9A: Native Gel Separation of LDH Isozymes (comprehensive). Experiment #9B: SDS-Polyacrylamide Gel Electrophoresis (short version). Experiment #9C: SDS-Polyacrylamide Gel Electrophoresis (comprehensive). 10. Western Blots. 10/1 Western Blot Theory. 10/2 Antibodies/10/3 Color development. 10/4 Blocking and Washing. 10/5 Why Is This Important'. Experiment #10: Western Blot of Serum Proteins. Experiment #10a ; Western Blot of LDH. 11. Restriction Enzymes. Restriction nucleases. Restriction maps. Agarose Gel Separation of DNA. Staining DNA. Phage DNA. Why Is This Important'. Experiment #11: Analysis of DNA Restriction Fragments. 12. Cloning and Expression of Foreign Proteins. Recombinant DNA. Vectors. Foreign DNA. Restriction Enzymes. Cell Lines. Transformation. Selection. Expression. Why Is This Important'. Experiment #12: Cloning and Expression of Barracuda LDH-A. 13. Polymerase Chain Reaction. Amplification of DNA. Taq Polymerase. Primers. Changing Restriction Sites. Why Is This Important'. Experiment #13: PCR of Barracuda LDH-A.

Please Wait