Description: This Methods in Enzymology volume deals with the utility of green fluorescent protein (GFP). It documents many diverse uses for this interesting molecule in disciplines that broadly span biology. The methods presented include shortcuts and conveniences not included in previously published sources. The techniques are described in a context that allows comparisons to other related methodologies -- such comparisons are valuable to readers who must adapt existing procedures to new systems. Also, so far as possible, methodologies have been presented in a manner that stresses their general applicability and potential limitations. This volume provides a substantial and current overview of the extant methodology in the field and a view of its rapid development.

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