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Immobilized Affinity Ligand Techniques cover
  • ISBN: 9780123423306
  • ISBN10: 0123423309

Immobilized Affinity Ligand Techniques

by Hermanson, Greg T. A Smith, Paul K.

  • List Price: $111.00
  • Binding: Paperback
  • Publisher: Academic Press
  • Publish date: 08/01/1992
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Description
This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Key Features* Matrix activation* Ligand immobilization* Binding and elution of target molecules* Enzyme catalysis on solid supports* Analytical affinity chromatography* Isolation/purification of antibodies* Preparation of antibody fragments* Immunoaffinity chromatography* Immobilization of nucleic acids* Use of immobilized ligands for removal of trace contaminantsPractical advice on choosing: * Matrices* Spacers* Methods of activation and couplingBackground information and insights on: * Affinity interactions* The ease and power of affinity chromatography* Attaching molecules to insoluble supports* Matrices currently in use* Over 20 methods of activation* Spacers* Extensive References
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Seller Condition Comments Price  
New Leaf Books

Location: Wichita, KS
Very Good
Used-Very Good A well-cared for item that has seen limited use but remains in
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$66.82
Graham Sadler Books

Location: Denver, CO
Like New
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8vo-over 7¾"-9¾" tall. Previous owner name stamped in book.
$67.97
Salish Sea Books

Location: Bellingham, WA
Very Good
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0123423309 Very Good++; Softcover, Plastic-Comb Binding; 1992, Academic
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$88.60
Hippo Books

Location: Toledo, OH
Very Good
May have shelf wear or edge rubbing. Pages are clean and free of textual notes or other markings.
$113.91