Description: This practical lab manual is designed for any molecular biology or clinical lab wishing to use 2-D to analyze the global protein content of cells or tissue. The book's contributors include pioneers in the development of high-resolution 2-D gels, immobilized pH gradients, computer analysis, and mass spec-based protein identification methodologies. The researcher is guided through the complete process of using 2-D protein gels from making the protein extract to finally identifying the proteins. It includes protocols for working with all of the model organisms including bacteria, yeast, and human. As well as protocols for comparing results with Internet-based 2-D databases
-- Includes chapters by those who pioneered the field including Klose and Gorg, as well as from contemporary leaders including Rabilloud, Hochstrasser, Appel, Yates, and Mann

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