Description: This course manual instructs students in recombinant DNA techniques and other essential molecular biology techniques in the context of projects. The project approach inspires and captivates students; it involves them in the scientific experience, providing continuity to laboratory bench time and an understanding of the principles underlying the techniques presented. < B> Molecular Biology< /B> is a must for any department, operating under budgetary constraints that offers or plans to offer a course in molecular cloning. Key Features* Includes a glossary of over 200 terms important for understanding molecular biology* Uses an inexpensive source of eukaryotic cells - great for schools on a budget* Includes Methods Locator that provides instant access to the latest methods* Contain clearly written, easy-to-follow, student-tested instructions: * Sterile techniques* Phage titration* Gel electrophoresis of DNA* Restriction enzyme digestion* Plasmid isolation* Transformation of < I> E. Coli< /I> * Recombinant DNA cloning* Nick translation labeling * Nonradioactive primer labelling* Nonradioactive DNA detection* Southern blotting* Colony hybridization* Purification of plant DNA* RNA purification* Northern blotting* Purification of poly A+ RNA* Polymerase chain reaction (PCR)

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