Description: Sampling, Characterization, and Handling ex vivo of Tissue Specimens: M.T. Bayliss, Introduction. D.L. Gardner, Articular Cartilage Samples. T.R. Oegema, Selection and Classification of Human Articular Cartilage. A. Maroudas, R. Schneiderman, C. Weinberg, and G. Grushko, Choice of Specimens in Comparative Studies Involving Human Femoral Head Cartilage. S. Roberts, Sampling of the Intervertebal Disc. E.B. Hunziker, Tissue Sampling and Preservation for Morphological Studies. Extraction, Separation, and Analysis of Matrix Constituents: D.R. Eyre, Introduction. D.R. Eyre, J.J. Wu, C. Niyibizi, and I. Chun, The Cartilage Collagens: Analysis of Their Cross-Linking Interactions and Matrix Organization. S. Ayad, A. Marriott, K. Morgan, C. Cummings, A.P.L. Kwan, A.P. Mould, and M.E. Grant, Mammalian Cartilage Collagens: Identification of Their Forms in Vivo. C.A. McDevitt, Composite Agarose-Acrylamide Electrophoresis of Proteoglycans and Large Protein Complexes. V. Stanescu, Analytical and Preparative Electrophoresis of Proteoglycan Monomers in Agarose Submerged Cells. J.C. Pita and F.J. Muller, Centrifugal Methodologies to Study the Proteoglycans from Articular Cartilage. M. van der Rest, E. de Miguel, Q. Nguyen, B. Dublet, J.S. Mort, and P.J. Roughley, Microsequencing of Cartilage Components. M. Bayliss, Extraction and Purification of Proteoglycan and Hyaluronic Acid from Human Articular Cartilage. H. Muir, Comments. Morphology of Cartilage: R.A. Stockwell, Introduction. D.L. Gardner, K. Oates, D.M. Lawton, J.G. Pidd, and J.F.S. Middleton, Methods for the Study of Cartilage by Low Temperature Scanning Electron Microscopy and Related Techniques. R. Stanescu, Electron Microscopic Study of the Articular Surface Using Cationized Ferritin Labeling. N.D. Broom, New Experimental Approaches to the Understanding of Structure/Function Relationships in Articular Cartilage. E.B. Hunziker, The Application of Recent Stereological Methods in Articular Cartilage Research. C.A. Poole, Chondrons Extracted from Articular Cartilage: Methods and Applications. Chondrocyte Culture: P.D. Benya, Introduction and Survey of Techniques for Chondrocyte Culture. M. Aydelotte, B.L. Schumacher, and K.E. Kuettner, Sub-Population of Articular Chondrocytes Cultured in Agarose Gel. C. Bassleer, Y. Henrotin, R. Bassleer, P. Franchimont, A Three-Dimensional Culture Model for Studying Human Chondrocyte Functions. S. Thenet and M. Adolphe, Immortalization of Chondrocytes in Culture. Z. Nevo, D. Robinson, N. Halperin, and S. Edelstein, Culturing Chondrocytes for Implantation. References. Short-Term and Long-Term Explant Culture of Cartilage: C.J. Handley, C.K. Ng, and A.J. Curtis, Introduction. V.C. Hascall, F.P. Luten, A.H.K. Plaas and J.D. Sandy, Steady State Metabolism of Proteoglycans in Bovine Articular Cartilage. J. Tyler and Y. Sawyer, Cartilage Explant Cultures: A Model System for the Analysis of Matrix Degradation. R.L.-Y. Sah, Y-J. Kim, and A.J. Grodzinsky, The Effect of Mechanical Compression on Cartilage Metabolism. A. Maroudas and R. Schneiderman, Effects of Mechanical and Osmotic Pressure on the Rate of Glycosaminoglycan Synthesis in the Human Adult Femoral Head Cartilage. B. Johnstone, Explant Culture of the Intervertebral Disc. References. Use of Radioisotopes to Study Metabolism of Matrix Macromolecules: V.C. Hascall, Introduction. V.C. Hascall, M. Yanagishita, A. Salustri, and T.I. Morales, The Use of Radiolabelled Glucosamine as a Precursor for Measuring Hyaluronan Synthesis. R.M. Mason, Assessment of Turnover of Proteoglycans in Vivo.

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