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DNA Isolation and Sequencing

by Bruce A. Roe; Judy S. Crabtree; A. Khan

  • ISBN: 9780471963240
  • ISBN10: 0471963240

DNA Isolation and Sequencing

by Bruce A. Roe; Judy S. Crabtree; A. Khan

  • List Price: $56.00
  • Binding: Paperback
  • Publisher: John Wiley & Sons
  • Publish date: 05/01/1996
  • ISBN: 9780471963240
  • ISBN10: 0471963240
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Description: Partial table of contents:GENERAL METHODS.Restriction Digestion.Agarose Gel Electrophoresis.Bacterial Cell Maintenance and Storage.RANDOM SUBCLONE GENERATION.Sonication.DNA Ligation.METHODS FOR DNA ISOLATION.Large Scale Double-Stranded DNA Isolation.Miniprep Double-Stranded DNA Isolation.METHODS FOR DNA SEQUENCING.Radiolabeled Sequencing Gel Preparation, Loading and Electrophoresis.Flourescent DNA Sequencing.ADDITIONAL METHODS.Polymerase Chain Reaction (PCR).Purification of PCR Fragments for Cloning.Synthesis and Purification of Oligonucleotides.Appendices.Index.
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