Description: An informative collection of techniques and protocols for characterizing and utilizing DNA repair activities and enzymes from some of the leading names in the DNA repair field. This collection of protocols is ideal for both the novice in the field and to the experienced applied researcher. The book is split into two sections. The first section deals with the classic identification, purification and characterization of DNA repair enzymes. A wide variety of repair pathways are touched upon including base excision repair, mismatch repair and recombination repair and each chapter explores the activities and characterization of specific DNA repair proteins. The second section of the book provides several protocols for the applied use of several DNA repair proteins in the latest molecular biology techniques including mutation detection, cloning and genome diversification. Therefore the book provides the novice with a practical selection of the main DNA repair protocols and provides the applied researcher with novel protocols in molecular biology and molecular genetics.

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