Description: Generation of Monoparental Embryos for Investigation into Genomic Imprinting.- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting.- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome.- Production of YAC Transgenic Mice by Pronuclear Injection.- A Transgenic Approach to Studying Imprinted Genes.- Methylation-Sensitive Genome Scanning.- Subtraction-Hybridization Method for the Identification of Imprinted Genes.- Identification of Imprinted Loci by Methylation.- Ribonuclease Protection.- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression.- Allele-Specific In Situ Hybridization (ASISH).- RNA-FISH to Analyze Allele-Specific Expression.- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes.- Southern Analysis Using Methyl-Sensitive Restriction Enzymes.- A PCR-Based Method for Studying DNA Methylation.- Bisulfite-Based Methylation Analysis of Imprinted Genes.- Direct Analysis of Chromosome Methylation.- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs.- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation.- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon.- Probing Chromatin Structure with Nuclease Sensitivity Assays.- Examining Histone Acetlylation at Specific Genomic Regions.- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis.- Reconstitution of Chromatin In Vitro.- Genomic Imprinting in Plants.

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