Description: Dedication. List of contributors. Tn917 transponson mutagenesis and marker rescue of interrupted genes of Streptococcus mutans; D.G. Cvitkovitch, et al. Site-specific homologous recombination mutagenesis in group B streptococci; H.H. Yim, C.E. Rubens. Targeted mutagenesis of enterococcal genes; X. Qin, et al. A lactococcal pWV01-based integration toolbox for bacteria; K. Leenhouts, et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion; W.M. McShan, et al. Streptococcal integration vectors for gene inactivation and cloning; Lin Tao. Induction of transformation in streptococci by synthetic competence stimulating peptides; P. Gaustad, D.A. Morrison. Characterization of the lactococcal conjugative element pRS01 using IS946-mediated mutagenesis; D.A. Mills, et al. Use of electroportation in genetic analysis of enterococcal virulence; H. Hirt, et al. Genetic transfer methods for Streptococcus sobrinus and other oral streptococci; D.J. LeBlanc, et al. Isolation of enterococcal antigen-encoding genes from genomic libraries; Yi Xu, et al. A simple microtiter plate screening assay for bacterial invasion or adherence; V. Nizet, et al. End-probing: A non-radioactive approach to mapping transponson insertions; M.H. Lee, et al. A method for mapping phage-inducible promotors for use in bacteriophage-triggered defense systems; G.M. Djordjevic, T.R. Klaenhammer. Secretion of heterologous proteins by genetically engineered Streptococcus gordonii; T. Shiroza, H. Kuramitsu. Examination of streptococcal gene expression in the mammalian environment; W.T. Grey, et al. Analysis of adherence-associated gene expression in Streptococcus parasangusis: A method for RNA isolation; E.H. Froeliger, P. Fives-Taylor. Development of an integrative, lacZ transcriptional-fusion plasmid vector for Streptococcus mutans and its use to isolate expressed genes; F. Peruzzi, et al. Use of proteomics and PCR to elucidate changes in protein expression in oral streptococci; R.G. Quivey Jr, et al. The use of continuous flow bioreactors to explore gene expression and physiology of suspended and adherent populations of oral streptococci; R.A. Burne, Yi-Ywan M. Chen. In vitro systems for investigating group B streptococcal: host cell and extracellular matrix interactions; S.B. Winram, et al. The rat model of endocarditis; C.L. Munro. Lipoproteins and other cell-surface associated proteins in streptococci; R. McNab, H.F. Jenkinson. Growth of Streptococcal mutans in an iron-limiting medium; G.A. Spatafora, M.W. Moore. Identification of oral streptococci using PCR-based, reverse-capture, checkerboard hybridization; B.J. Paster, et al. Pulsed-field gel electrophoresis as an epidemiologic tool for enterococci and streptococci; J.E. Patterson, C.C. Kelly. Cell-based panning as a means to isolate phage display Fabs specific for a bacterial surface protein; A.E. Stephenson, et al. Instructions for authors. Announcement.

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