Description: The Second Edition has been divided into two complementary parts: Recombinant DNA, and Gene Analysis. Together they comprise a comprehensive compendium of the most up-to-date methods available in molecular biology, covering enzymes used for restriction, methylation and modification of nucleic acids, cloning vectors, hosts, genomes and genes, as well as techniques such as blotting and hybridization, DNA sequencing, PCR methodology, labeling and separation of nucleic acids. This volume covers the general information that is relevant to most, if not all, molecular biology experiments. The Second Edition also provides useful information on general chemicals and reagents and safety considerations, which are clearly presented, easily accessible, and complemented by excellent diagrams. This book presents key information on techniques, sources of reagents, equipment, gene constructs, cloning vectors, enzymes, bacteria, phages, genomes and genes used worldwide by anyone using molecular biological techniques.

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