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Mycobacterium Tuberculosis Protocols

by Tanya Parish

  • ISBN: 9780896037762
  • ISBN10: 0896037762

Mycobacterium Tuberculosis Protocols

by Tanya Parish

  • List Price: $229.00
  • Binding: Hardcover
  • Publisher: Humana Pr Inc
  • Publish date: 05/01/2001
  • ISBN: 9780896037762
  • ISBN10: 0896037762
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Description: This book provides detailed protocols for studying the molecular biology of the pathogen, Mycobacterium tuberculosis, and its interactions with host cells. It covers the entire spectrum of techniques from perspectives on storage of strains and safety issues to the application of the latest functional genomics strategies. With the completion of the genome sequence of the H37Rv strain in 1998 we are now able to predict every protein and RNA made by this pathogen. Also included are the latest mutagenesis methodologies that allow for functional studies. This volume provides the techniques used to study the functional genomics of the pathogen and integrates them with other disciplines including immunology, cell biology, ad biochemistry. Chapters cover proteome analysis, and the analysis of whole genome microarrays. Also covered is a new method for detecting bacteria and identifying drug resistance using an affordable sensitive phage-based technique. Finally, the authors provide a valuable description of the main M. tuberculosis genome resources appearing on the Internet, making this an essential reference for all those working on this deadly pathogen.
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