Description: Leading scientists including Fred H. Gage, Lloyd A. Greene, Paul Fernyhough, and Ronald G. Wiley detail their special expertise by providing a wide variety of techniques utilized in neurotrophin research. These powerful procedures have been specifically developed to address particular issues within the neurotrophic factor field. The book is divided into four section dealing with protein, RNA, recombinant, and in vivo techniques. Novel methods that are not available elsewhere cover transgenic cell lines and animals. Quantitative and histological methods for the analysis of neurotrophin mRNA are also included. Other procedures include the increasingly popular use of immunotoxins to study the effects of elimination of a single class of neurons and the essential stereological method for estimation of neuronal numbers. Because of the wide range of protocols and the extensive contributions of the authors, Neurotrophin Protocols will be of essential value and utility to both postgraduate students and established investigators alike.

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