Description: Techniques for Free Radical Derived Biomarkers.- Human Xanthine Oxidoreductase Determination by a Competitive ELISA.- Simultaneous Determination of Polyunsaturated Fatty Acids and Corresponding Monohydroperoxy and Monohydroxy Peroxidation Products by HPLC.- Determination of Products of Lipid Oxidation by Infrared Spectroscopy.- Detection of Docosahexaenoic Acid Hydroperoxides in Retina by Gas Chromatography/Mass Spectrometry.- Detection of Lipid Hydroperoxide-Derived Protein Modification with Polyclonal Antibodies.- Techniques for Determining the Metabolic Pathways of Eicosanoids and for Evaluating the Rate-Controlling Enzymes.- Mass Spectrometric Quantification of F2-Isoprostanes as Indicators of Oxidant Stress.- Formation of Apolipoprotein AI-AII Heterodimers by Oxidation of High-Density Lipoprotein.- Detection of Certain Peroxynitrite-Induced DNA Modifications.- Hydroxyl and 1-Hydroxyethyl Radical Detection by Spin Trapping and GC-MS.- Analysis of Aliphatic Amino Acid Alcohols in Oxidized Proteins.- Rapid Determination of Glutamate Using HPLC Technology.- A Rapid Method for the Quantification of GSH and GSSG in Biological Samples.- Protein Carbonyl Measurement by ELISA.- N ?-(carboxymethyl)lysine (CML) as a Biomarker of Oxidative Stress in Long-Lived Tissue Proteins.- Measurement of S-Glutathionated Hemoglobin in Human Erythrocytes by Isoelectric Focusing Electrophoresis.- Oxidation of Cellular DNA Measured with the Comet Assay.- Measurement of DNA Double-Strand Breaks with Giant DNA and High Molecular-Weight DNA Fragments by Pulsed-Field Gel Electrophoresis.- Evaluation of Antibodies Against Oxygen Free Radical-Modified DNA by ELISA.- Techniques for Antioxidant Biomarkers.- Simultaneous Analysis of Multiple Redox-Active Metabolites from Biological Matrices.-Determination of Uric Acid in Urine by Fast-Scan Voltammetry (FSV) Using a Highly Activated Carbon Fiber Electrode.- Measurement of ?-Tocopherol Turnover in Plasma and in Lipoproteins Using Stable Isotopes and Gas Chromatography/Mass Spectrometry.- Analysis of Tocotrienols in Different Sample Matrixes by HPLC.- Measurement of ?-Carotene15,15?-Dioxygenase Activity by Reverse-Phase HPLC.- Ubiquinol/Ubiquinone Ratio as a Marker of Oxidative Stress.- Catechol- and Pyrogallol-Type Flavonoids.- Pyruvate Dehydrogenase Complex as a Marker of Mitochondrial Metabolism.- Ceruloplasmin Detection by SDS-PAGE, Immunoblotting, and In Situ Oxidase Activity.- Metallothionein Determination by Isocratic HPLC with Fluorescence Derivatization.- Quantification of Oxidized Metallothionein by a Cd-Saturation Method.- Fractionation of Herbal Medicine for Identifying Antioxidant Activity.- Designing Safer (Soft) Drugs by Avoiding the Formation of Toxic and Oxidative Metabolites.- Statistical Correction of the Area Under the ROC Curve in the Presence of Random Measurement Error and Applications to Biomarkers of Oxidative Stress.

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