Description: This third edition of a best-selling and highly praised collection of unfailingly reproducible methods used in the neuroscience laboratory. The chapters carried over from the second edition have been extensively revised and updated, while 11 new chapters have been added which come from well-established tissue culture laboratories with proven records. The new chapters deal with preparation of substrata, use of serum-free media, maintaining hybridomas, and the production and purification of monoclonal antibodies. Also included are new and extensive chapters on the isolation, growth, and characterization of neural stem cells and glia progenitor cells. This book's unique wealth of practical detail and its inclusion of a wide range of proven tissue culture systems having many applications ensure that it will remain "a valuable resource that will find its place on many laboratory shelves (Trends in Biotechnology)"

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