Description: < B> Pulsed Field Gel Electrophoresis: A Practical Guide< /B> is the first laboratory manual to describe the theory and practice of this technique. Based on the authors' experience developing pulsed field gel instruments and teaching procedures, this book provides everything a researcher or student needs to know in order to understand and carry out pulsed field gel experiments. Clear, well-tested protocols assume only that users have a basic familiarity with molecular biology. Thorough coverage useful data, theory, and applications ensures that this book is also a lasting resource for more advanced practitioners of pulsed field gels. Key Features* Reviews all types of pulsed field gel electrophoresis* Describes all commercially available systems and summarizes advantages and limitations of each* Includes step-by-step protocols for sample preparation and analysis* Illustrated with photographs that depict * How to run gels* What the results should look like* What they look like when they go wrong* Covers applications to a wide range of organisms* Includes bibliography of over 900 publications and cross-referenced by topic, application, and organism

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