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Techniques in Protein Chemistry VIII cover
  • ISBN: 9780124735576
  • ISBN10: 0124735576

Techniques in Protein Chemistry VIII

by Marshak, Daniel R.

  • List Price: $130.00
  • Binding: Hardcover
  • Publisher: Academic Press
  • Publish date: 03/01/2007
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Foreword. Preface. Acknowledgments. Primary Structural Analysis: Protein Sequencing Using Microreactors and Capillary Electrophoresis with Thermo-optical Absorbance Detection: Enhancement of Concentration Limits of Detection in Capillary Electrophoresis: Examples of On-Line Sample Preconcentration, Cleanup, and Microreactor Technology in Protein Characterization. Sequencing MHC Class I Peptides Using Membrane Preconcentration-Capillary Electrophoresis Tandem Mass Spectrometry (mPC-CE-MS/MS). Nano-electrospray Mass Spectrometry and Edman Sequencing of Peptides and Proteins Collected from Capillary Electrophoresis. Characterization of a Recombinant Hepatitis E Protein Vaccine Candidate by Mass Spectrometry and Sequencing Techniques. Comparison of the High Sensitivity and Standard Versions of Applied Biosystems Procise TM 494 N-Terminal Protein Sequencers Using Various Sequencing Supports. Evaluation of ABRF-96SEQ: A Sequence Assignment Exercise. Internal Protein Sequencing of SDS-PAGE-Separated Proteins: Optimization of an In Gel Digest Protocol. A Strategy to Obtain Internal Sequence Information from Blotted Proteins after Initial N-terminal Sequencing. Internal Protein Sequencing of SDS PAGE-Separated Proteins: A Collaborative ABRF Study. Physical and Chemical Analysis: Chromatographic Determination of Extinction Coefficients of Non-Glycosylated Proteins Using Refractive Index (RI) and UV Absorbance (UV) Detectors: Applications for Studying Protein Interactions by Size Exclusion Chromatography with Light-Scattering, UV, and RI Detectors. Single Alkaline Phosphatase Molecule Assay by Capillary Electrophoresis Laser-Induced Fluorescence Detection. A New Centrifugal Device Used in Sample Clean-up and Concentration of Peptides. Sample Preparation Using Synthetic Membrances for the Study of Biopolymers by Matrix Assisted Laser Desorption/Ionization Mass Spectrometry. Use of LC/MS Peptide Mapping for Characteriation of Isoforms in 15 N-Labeled Recombinant Human Leptin. Hyphenated HPLC Methodology for the Resolution and Elucidation of Peptides from Proteolytic Digests. Detecting and Identifying Active Compounds from a Combinatorial Library Using Iasys and Electrospray Mass Spectrometry. Amino Acid Analysis of Unusual and Complex Samples Based on 6-Aminoquinolyl-N-hydroxysuccinimidyl Carbamate Derivatization. Development of a Method for Analysis of Free Amino Acids from Physiological Samples Using a 420A ABI/PE Amino Acid Analyzer. Quantitation and Identification of Proteins by Amino Acid Analysis: ABRF-96AAA Collaborative Trial Chemical Modification: Nonaqueous Chemical Modification of Lyophilized Proteins Reaction of HIV-1 NC p7 Zinc Fingers with Electrophilic Reagents. The Identification and Isolation of Reactive Thiols in Ricin A-Chain and Blocked Ricin Using 2-(4'-Maleimidylanilino)naphthalene-6-sulfonic Acid. Inactivation of the Human Cytomegalovirus Protease by Diisopropylfluorophosphate. Studies on the Status of Arginine Residues in Phospholipase A 2 from Naja naja atra (Taiwan cobra) Snake Venom. Selective Reduction of the Intermolecular Disulfide Bridge in Human Glial Cell Lined-Derived Neurotrophic Factor Using Tris-(2-Carboxyethyl)Phosphine. Effects of Surface Hydrophobicity on the Structural Properties of Insulin. The Effects of in Vitro Methionine Oxidation on the Bioactivity and Structure of Human Keratinocyte Growth Factor. Posttranslational and Other Modifications: Effects of Enzyme Glycosylation on the Chemical Step of Catalysis, as Probed by Hydrogen Tunneling and Enthalpy of Activation. Profile Analysis of Oligosaccharides from Glycoproteins by PMP Labeling. Comparison of Chemical and Enzymatic Release Methods Using RP-HPLC and Mass Spectrometry. Positive Identification of Glycosylation Sites in Proteins and Peptides Using a Modified Beckman LF 3600
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