Description: 1. The two-hybrid system: A personal viewI. The Two-Hybrid System2. Searching for interacting proteins with the two-hybrid system I3. Searching for interacting proteins with the two-hybrid system II4. Searching for interacting proteins with the two-hybrid system III5. Constructing an activation domain fusion libraryII. Analyzing Interactions with the Two-Hybrid System6. Obtaining structural information about protein complexes with the two-hybrid system7. The reverse two-hybrid system8. Dissecting the biological relevance of multiple interactions9. Investigation of ligand/receptor interactions and the formation of ternary complexes10. Two-hybrid analysis of protein-protein interactions in the yeast pheromone response pathway11. Two-hybrid screening and the cell cycle12. Two-hybrid analysis of genetic regulatory networksIII. Variations on the Two-Hybrid Theme13. Mammalian cells as hosts for two-hybrid studies of protein-protein interaction14. Detection of protein-protein interactions dependent on post-translational modifications15. Protein interactions mediated by small molecule ligands16. Use of a combinatorial peptide library in the two-hybrid assayIV. Related Methodologies17. Isolation of DNA-binding proteins using a one-hybrid genetic screen18. A three-hybrid system to detect and analyze RNA-protein interactions in vivo19. Split ubiquitin: a sensor of protein interactions in vivo

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