Description: Neural and Neuromuscular Systems: Hippocampal Slices. Organotypic Neural Cultures. Reaggregate Cells for Neurotoxicological Studies. Cytotoxicity in Murine Neocortical Cell Culture. Cultures of Adult Trigeminal Ganglion Neurons. Adrenomedullary Chromaffin Cells: A Well-Characterized Model System for Toxicological Studies. PC 12 Cells. Ocular Systms: Corneal Epithelial and Endothelial Cell Cultures. Isolation and Culture of Retinal Epithelium. Respiratory System: Isolation and Culture of Type II Alveolar Epithelial Cells. Isolation of Nonciliated Bronchiolar (Clara) Epithelial Cells from Mouse Lung. Rat Tracheal Epithelial Cell Cultures for Studies in Toxicology and Carcinogenicity. Cardiovascular System: Preparation of Primary Cultures of Postnatal Rat Myocardial Cells for Toxicological Studies. Aortic Endothelial and Smooth Muscle Cell Cultures. Vessel Cylinders. Gastrointestinal System: Gastric Mucosal Cell Culture for Toxicological Study. Small Intestinal Enterocytes. Maintenance and Characterization of an Organ-Culture System for Colonic Mucosa from the Rate. Liver Slices:Precision-Cut Rate Liver Slices in Dynamic Organ Culture for Structure-Toxicity Studies. Liever: Hepatocytes: Isolation of Hepatocytes By Collagenase Perfusion. Preparation of Primary Monolayer Cultures of Postnatal Rat Liver Cells for Hepatotix Assessment of Xenobiotics. Isolation and Culture of Hepatocytes from Different Laboatory Species. Isolation of Human Hepatocytes By Biopsy Perfusion Methods. Human Hepatocyte Cultures. Liver: Other Cell Systems: Hepatocyte and Spleen Cell Systems. Isolation and Culture of Hepatic Non-Parenchymal Cells. Proliferating Lines of Rat Liver Epithelial Cells. Isolationa Nd Culture of Liver Epithelial Cells from Carcinogen-Treated Rats. Kidney: Proximal Tubule Fragments: Proximal Renal Tubule Preparations Isolated from Rat or Rabbit Kidneys Without the Use of Collagenase. Suspension Culture of Rabbit Renal Proimal Tubules. Perfusion Method for Isolation of Renal Proximal Tubules from Several Species. Isolation and Maintenance of Milligram Quantities of Rabbit Renal Proximal Straight and Convoluted Tubules. Kidney: Cell Culture: Rabbit Cortical Epithelial Cells. Culture of Rat Kidney Proximal Tubule Epithelial Cells in Defined Medium. Primary Culture of Rat Renal Medullary Interstitial Cells. Purification of Renal Cortical Cell Populations By Percoll Density-Gradient Centrifugation. Renal Cell Lines Derived from Rabbit and Pig Kidney. Reproductive and Developmental Systems: Whole Embryo Culture: Organogenesis of Rodent Embryos in Vitro. The Preparation and Use of Sertoli-Germ Cell Co-Cultures Fro 28-Day-Old Rates. Immune System. Isolation and Jn Vitro Culture of Mono-Nuclear Phagocytes. T-Lymphocyte Assays. B Lymphocytes: Comparison of Murine and Human B-Cell Immunocompetence. Bone Marrow Stromal Cell Cultures. Skin: Penetration Studies Using Excised Human and Animal Skin. Porcine Skin Flaps. Keratinocytes. Subject Index.

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